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Recombinant Human EPHX2 293 Cell Lysate

Cat.No. : EPHX2-6585HCL
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Description : Antigen standard for epoxide hydrolase 2, cytoplasmic (EPHX2) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : EPHX2 epoxide hydrolase 2, cytoplasmic [ Homo sapiens ]
Official Symbol : EPHX2
Synonyms : EPHX2; epoxide hydrolase 2, cytoplasmic; epoxide hydrolase 2; epoxide hydratase; epoxide hydrolase, soluble; epoxide hydrolase 2, cytosolic; CEH; SEH;
Gene ID : 2053
mRNA Refseq : NM_001256482
Protein Refseq : NP_001243411
MIM : 132811
UniProt ID : P34913
Chromosome Location : 8p21
Pathway : Arachidonate Epoxygenase / Epoxide Hydrolase, organism-specific biosystem; Arachidonic acid metabolism, organism-specific biosystem; Arachidonic acid metabolism, conserved biosystem; Metabolic pathways, organism-specific biosystem; Peroxisome, organism-specific biosystem; Peroxisome, conserved biosystem;
Function : epoxide hydrolase activity; hydrolase activity; metal ion binding; protein homodimerization activity;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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01/30/2023

    The product brochure is very detailed and has comprehensive basic information about the EPHX2 protein.

    11/09/2022

      The staff actively communicated with me and solved many technical problems.

      10/18/2022

        Very professional customer service, fully understanding the needs of the customer and giving professional advice.

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