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Recombinant Human ENC1 293 Cell Lysate

Cat.No.: ENC1-6603HCL
Description: Antigen standard for ectodermal-neural cortex (with BTB-like domain) (ENC1) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: ENC1 ectodermal-neural cortex 1 (with BTB-like domain) [ Homo sapiens ]
Official Symbol: ENC1
Synonyms: ENC1; ectodermal-neural cortex 1 (with BTB-like domain); NRPB; ectoderm-neural cortex protein 1; ENC 1; kelch like 37 (Drosophila); KLHL37; PIG10; TP53I10; kelch-like 35; kelch-like protein 37; p53-induced gene 10 protein; nuclear matrix protein NRP/B; tumor protein p53 inducible protein 10; nuclear restricted protein, BTB domain-like (brain); CCL28; ENC-1; KLHL35; FLJ39259; FLJ57577;
Gene ID: 8507
mRNA Refseq: NM_001256574
Protein Refseq: NP_001243503
MIM: 605173
UniProt ID: O14682
Chromosome Location: 5q13
Function: actin binding;

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