"EIF2B5" Related Products

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Recombinant Human EIF2B5 293 Cell Lysate

Cat.No.: EIF2B5-6669HCL
Description: Antigen standard for eukaryotic translation initiation factor 2B, subunit 5 epsilon, 82kDa (EIF2B5) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: EIF2B5 eukaryotic translation initiation factor 2B, subunit 5 epsilon, 82kDa [ Homo sapiens ]
Official Symbol: EIF2B5
Synonyms: EIF2B5; eukaryotic translation initiation factor 2B, subunit 5 epsilon, 82kDa; eukaryotic translation initiation factor 2B, subunit 5 (epsilon, 82kD); translation initiation factor eIF-2B subunit epsilon; EIF 2B; EIF2Bepsilon; eIF-2B GDP-GTP exchange factor subunit epsilon; CLE; CACH; LVWM; EIF-2B;
Gene ID: 8893
mRNA Refseq: NM_003907
Protein Refseq: NP_003898
MIM: 603945
UniProt ID: Q13144
Chromosome Location: 3q27.3
Pathway: Cap-dependent Translation Initiation, organism-specific biosystem; Eukaryotic Translation Initiation, organism-specific biosystem; Gene Expression, organism-specific biosystem; Metabolism of proteins, organism-specific biosystem; MicroRNAs in cardiomyocyte hypertrophy, organism-specific biosystem; RNA transport, organism-specific biosystem; RNA transport, conserved biosystem;
Function: contributes_to guanyl-nucleotide exchange factor activity; guanyl-nucleotide exchange factor activity; guanyl-nucleotide exchange factor activity; protein binding; transferase activity; contributes_to translation initiation factor activity; translation initiation factor activity; translation initiation factor binding;

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