"EFS" Related Products

Recombinant Human EFS 293 Cell Lysate

Cat.No.: EFS-6699HCL
Description: Antigen standard for embryonal Fyn-associated substrate (EFS), transcript variant 2 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: EFS embryonal Fyn-associated substrate [ Homo sapiens ]
Official Symbol: EFS
Synonyms: EFS; embryonal Fyn-associated substrate; Cas scaffolding protein family member 3; CASS3; EFS1; EFS2; HEFS; SIN; signal transduction protein (SH3 containing); CAS3;
Gene ID: 10278
mRNA Refseq: NM_005864
Protein Refseq: NP_005855
MIM: 609906
UniProt ID: O43281
Chromosome Location: 14q11.2-q12
Function: SH3 domain binding; protein domain specific binding;
Products Types ◆ Recombinant Protein

Related Gene
EFS

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