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Recombinant Human EFEMP1 293 Cell Lysate

Cat.No. : EFEMP1-6705HCL
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  • Gene Information
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Description : Antigen standard for EGF-containing fibulin-like extracellular matrix protein 1 (EFEMP1), transcript variant 3 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : EFEMP1 EGF containing fibulin-like extracellular matrix protein 1 [ Homo sapiens ]
Official Symbol : EFEMP1
Synonyms : EFEMP1; EGF containing fibulin-like extracellular matrix protein 1; DHRD, EGF containing fibulin like extracellular matrix protein 1 , FBNL, fibrillin like; EGF-containing fibulin-like extracellular matrix protein 1; FBLN3; fibulin 3; MTLV; S1 5; FIBL-3; fibulin-3; extracellular protein S1-5; DHRD; DRAD; FBNL; MLVT; S1-5; FLJ35535; MGC111353;
Gene ID : 2202
mRNA Refseq : NM_001039349
Protein Refseq : NP_001034438
MIM : 601548
UniProt ID : Q12805
Chromosome Location : 2p16
Function : calcium ion binding; epidermal growth factor receptor binding; epidermal growth factor-activated receptor activity; growth factor activity; protein binding;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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