Recombinant Human DUSP10 293 Cell Lysate DUSP10-6784HCL

Recombinant Human DUSP10 293 Cell Lysate

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Recombinant Human DUSP10 293 Cell Lysate

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Cat.No. : DUSP10-6784HCL
Description : Antigen standard for dual specificity phosphatase 10 (DUSP10), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : DUSP10 dual specificity phosphatase 10 [ Homo sapiens ]
Official Symbol : DUSP10
Synonyms : DUSP10; dual specificity phosphatase 10; dual specificity protein phosphatase 10; MKP 5; MKP5; map kinase phosphatase 5; dual specificity phosphatase MKP-5; serine/threonine specific protein phosphatase; mitogen-activated protein kinase phosphatase 5; MKP-5;
Gene ID : 11221
mRNA Refseq : NM_007207
Protein Refseq : NP_009138
MIM : 608867
UniProt ID : Q9Y6W6
Chromosome Location : 1q41
Pathway : ATF-2 transcription factor network, organism-specific biosystem; MAPK signaling pathway, organism-specific biosystem; MAPK signaling pathway, organism-specific biosystem; MAPK signaling pathway, conserved biosystem; Regulation of p38-alpha and p38-beta, organism-specific biosystem;
Function : MAP kinase tyrosine/serine/threonine phosphatase activity; hydrolase activity; phosphoprotein phosphatase activity; protein tyrosine phosphatase activity;

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