"DPCD" Related Products

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Recombinant Human DPCD 293 Cell Lysate

Cat.No.: DPCD-6839HCL
Description: Antigen standard for deleted in primary ciliary dyskinesia homolog (mouse) (DPCD) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: DPCD deleted in primary ciliary dyskinesia homolog (mouse) [ Homo sapiens ]
Official Symbol: DPCD
Synonyms: DPCD; deleted in primary ciliary dyskinesia homolog (mouse); protein DPCD; DKFZP566F084; RP11 529I10.4; deleted in a mouse model of primary ciliary dyskinesia; RP11-529I10.4; DKFZp566F084;
Gene ID: 25911
mRNA Refseq: NM_015448
Protein Refseq: NP_056263
UniProt ID: Q9BVM2
Chromosome Location: 10q24.32

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