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Recombinant Human DOK7 293 Cell Lysate

Cat.No.: DOK7-6843HCL
Description: Antigen standard for docking protein 7 (DOK7), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: DOK7 docking protein 7 [ Homo sapiens ]
Official Symbol: DOK7
Synonyms: DOK7; docking protein 7; C4orf25, chromosome 4 open reading frame 25; protein Dok-7; Dok 7; FLJ33718; FLJ39137; downstream of tyrosine kinase 7; CMS1B; C4orf25; FLJ90556;
Gene ID: 285489
mRNA Refseq: NM_001164673
Protein Refseq: NP_001158145
MIM: 610285
UniProt ID: Q18PE1
Chromosome Location: 4p16.2
Function: insulin receptor binding; lipid binding; protein kinase binding;

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