"DNAI1" Related Products

Recombinant Human DNAI1 293 Cell Lysate

Cat.No.: DNAI1-6896HCL
Description: Antigen standard for dynein, axonemal, intermediate chain 1 (DNAI1) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: DNAI1 dynein, axonemal, intermediate chain 1 [ Homo sapiens ]
Official Symbol: DNAI1
Synonyms: DNAI1; dynein, axonemal, intermediate chain 1; dynein, axonemal, intermediate polypeptide 1; dynein intermediate chain 1, axonemal; CILD1; PCD; immotile cilia syndrome 1; dynein intermediate chain DNAI1; axonemal dynein intermediate chain 1; ICS; ICS1; MGC26204;
Gene ID: 27019
mRNA Refseq: NM_012144
Protein Refseq: NP_036276
MIM: 604366
UniProt ID: Q9UI46
Chromosome Location: 9p13.3
Pathway: Huntingtons disease, organism-specific biosystem; Huntingtons disease, conserved biosystem;
Function: motor activity;

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