"DIRC1" Related Products

Recombinant Human DIRC1 293 Cell Lysate

Cat.No.: DIRC1-6920HCL
Description: Antigen standard for disrupted in renal carcinoma 1 (DIRC1) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: DIRC1?disrupted in renal carcinoma 1 [?Homo sapiens?(human) ]
Official Symbol: DIRC1
Synonyms: DIRC1; disrupted in renal carcinoma 1; disrupted in renal carcinoma protein 1; disrupted in renal cancer protein
Gene ID: 116093
mRNA Refseq: NM_052952
Protein Refseq: NP_443184
MIM: 606423
UniProt ID: Q969H9
Chromosome Location: 2q33
Products Types ◆ Recombinant Protein

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