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Recombinant Human DHPS 293 Cell Lysate

Cat.No. : DHPS-6942HCL
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  • Gene Information
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Description : Antigen standard for deoxyhypusine synthase (DHPS), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : DHPS deoxyhypusine synthase [ Homo sapiens ]
Official Symbol : DHPS
Synonyms : DHPS; deoxyhypusine synthase; MIG13; migration inducing gene 13; migration-inducing gene 13; DS; DHS;
Gene ID : 1725
mRNA Refseq : NM_001930
Protein Refseq : NP_001921
MIM : 600944
UniProt ID : P49366
Chromosome Location : 19p13.2
Pathway : Hypusine synthesis from eIF5A-lysine, organism-specific biosystem; Metabolism of proteins, organism-specific biosystem; PTM: gamma carboxylation, hypusine formation and arylsulfatase activation, organism-specific biosystem; Post-translational protein modification, organism-specific biosystem; hypusine biosynthesis, organism-specific biosystem; hypusine biosynthesis, conserved biosystem;
Function : deoxyhypusine synthase activity; protein binding; transferase activity;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (5)

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Are there any resistance mechanisms against sulfonamides targeting DHPS? 04/30/2021

Yes, bacterial resistance to sulfonamides can arise due to mutations in DHPS that reduce the drug's binding affinity.

Can DHPS mutations affect the effectiveness of sulfonamides in treating bacterial infections? 06/26/2020

Yes, mutations in DHPS can lead to reduced effectiveness of sulfonamides, contributing to antibiotic resistance.

Are there any other clinical implications of DHPS beyond antibiotic treatment? 03/24/2020

DHPS mutations have been associated with resistance to sulfadoxine-pyrimethamine, a drug used to prevent and treat malaria.

In what clinical conditions are sulfonamides commonly used? 05/25/2018

Sulfonamides are used to treat urinary tract infections, respiratory tract infections, and other bacterial infections.

Can DHPS be a target for drug development beyond antibiotics? 05/16/2016

DHPS has potential as a target for developing new antiparasitic drugs due to its role in folate biosynthesis in parasites such as Plasmodium.

Customer Reviews (3)

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Reviews
12/26/2020

    Their expert team has been instrumental in addressing any challenges or concerns I have encountered during my experiments.

    02/14/2019

      Their prompt and comprehensive assistance has proven invaluable in overcoming obstacles and optimizing my experimental protocols.

      02/05/2019

        The DHPS protein boasts remarkable stability, allowing for extended storage without compromising its integrity or performance.

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