"DDX39A" Related Products

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Recombinant Human DDX39 293 Cell Lysate

Cat.No.: DDX39A-7008HCL
Description: Antigen standard for DEAD (Asp-Glu-Ala-Asp) box polypeptide 39 (DDX39) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: DDX39A?DEAD (Asp-Glu-Ala-Asp) box polypeptide 39A [?Homo sapiens?(human) ]
Official Symbol: DDX39A
Synonyms: DDX39A; DEAD (Asp-Glu-Ala-Asp) box polypeptide 39A; BAT1; DDXL; BAT1L; DDX39; URH49; ATP-dependent RNA helicase DDX39A; ATP-dependent RNA helicase DDX39A; DEAD box protein 39; nuclear RNA helicase URH49; UAP56-related helicase, 49 kDa; DEAD (Asp-Glu-Ala-Asp) box polypeptide 39; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 39; nuclear RNA helicase, DECD variant of DEAD box family; NP_005795.2; EC 3.6.4.13
Gene ID: 10212
mRNA Refseq: NM_005804
Protein Refseq: NP_005795
UniProt ID: O00148
Chromosome Location: 19p13.12
Function: ATP binding; helicase activity; poly(A) RNA binding; protein binding

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