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Recombinant Human DCTN2 293 Cell Lysate

Cat.No. : DCTN2-7042HCL
  • Specification
  • Gene Information
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Description : Antigen standard for dynactin 2 (p50) (DCTN2) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : DCTN2 dynactin 2 (p50) [ Homo sapiens ]
Official Symbol : DCTN2
Synonyms : DCTN2; dynactin 2 (p50); dynactin subunit 2; DCTN 50; RBP50; p50 dynamitin; dynactin complex 50 kD subunit; dynactin complex 50 kDa subunit; 50 kD dynein-associated polypeptide; 50 kDa dynein-associated polypeptide; DCTN50; DYNAMITIN;
Gene ID : 10540
mRNA Refseq : NM_006400
Protein Refseq : NP_006391
MIM : 607376
UniProt ID : Q13561
Chromosome Location : 12q13.3
Pathway : Cell Cycle, organism-specific biosystem; Cell Cycle, Mitotic, organism-specific biosystem; Centrosome maturation, organism-specific biosystem; G2/M Transition, organism-specific biosystem; Huntingtons disease, organism-specific biosystem; Huntingtons disease, conserved biosystem; Loss of Nlp from mitotic centrosomes, organism-specific biosystem;
Function : motor activity; protein binding;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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