"CYB5R1" Related Products


Recombinant Human CYB5R1 293 Cell Lysate

Cat.No.: CYB5R1-7144HCL
Description: Antigen standard for cytochrome b5 reductase 1 (CYB5R1) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: CYB5R1 cytochrome b5 reductase 1 [ Homo sapiens ]
Official Symbol: CYB5R1
Synonyms: CYB5R1; cytochrome b5 reductase 1; NAD(P)H:quinone oxidoreductase type 3, polypeptide A2 , NQO3A2; NADH-cytochrome b5 reductase 1; humb5R2; NAD(P)H:quinone oxidoreductase type 3 polypeptide A2; NAD(P)H:quinone oxidoreductase type 3, polypeptide A2; B5R1; B5R.1; NQO3A2;
Gene ID: 51706
mRNA Refseq: NM_016243
Protein Refseq: NP_057327
MIM: 608341
UniProt ID: Q9UHQ9
Chromosome Location: 1q32.1
Pathway: Amino sugar and nucleotide sugar metabolism, organism-specific biosystem; Amino sugar and nucleotide sugar metabolism, conserved biosystem;
Function: cytochrome-b5 reductase activity; oxidoreductase activity;

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