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Recombinant Human CTDSP2 293 Cell Lysate

Cat.No.: CTDSP2-7209HCL
Description: Antigen standard for CTD (carboxy-terminal domain, RNA polymerase II, polypeptide A) small phosphatase 2 (CTDSP2) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: CTDSP2 CTD (carboxy-terminal domain, RNA polymerase II, polypeptide A) small phosphatase 2 [ Homo sapiens ]
Official Symbol: CTDSP2
Synonyms: CTDSP2; CTD (carboxy-terminal domain, RNA polymerase II, polypeptide A) small phosphatase 2; carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase 2; conserved gene amplified in osteosarcoma; NLI interacting factor 2; nuclear LIM interactor interacting factor 2; OS4; PSR2; SCP2; small CTD phosphatase 2; protein OS-4; NLI-interacting factor 2; small C-terminal domain phosphatase 2; nuclear LIM interactor-interacting factor 2;
Gene ID: 10106
mRNA Refseq: NM_005730
Protein Refseq: NP_005721
MIM: 608711
UniProt ID: O14595
Chromosome Location: 12q14.1
Pathway: Activation of Chaperone Genes by XBP1(S), organism-specific biosystem; Activation of Chaperones by IRE1alpha, organism-specific biosystem; BMP receptor signaling, organism-specific biosystem; Coregulation of Androgen receptor activity, organism-specific biosystem; Diabetes pathways, organism-specific biosystem; Disease, organism-specific biosystem; Regulation of cytoplasmic and nuclear SMAD2/3 signaling, organism-specific biosystem;
Function: CTD phosphatase activity; hydrolase activity; metal ion binding; phosphoprotein phosphatase activity;

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