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Recombinant Human CSNK2B 293 Cell Lysate

Cat.No.: CSNK2B-7237HCL
Description: Antigen standard for casein kinase 2, beta polypeptide (CSNK2B) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: CSNK2B casein kinase 2, beta polypeptide [ Homo sapiens ]
Official Symbol: CSNK2B
Synonyms: CSNK2B; casein kinase 2, beta polypeptide; casein kinase II subunit beta; phosvitin; CK II beta; protein G5a; Casein kinase II beta subunit; alternative name: G5a, phosvitin; G5A; CK2B; CK2N; CSK2B; MGC138222; MGC138224;
Gene ID: 1460
mRNA Refseq: NM_001320
Protein Refseq: NP_001311
MIM: 115441
UniProt ID: P67870
Chromosome Location: 6p21.33
Pathway: ALK1 signaling events, organism-specific biosystem; Adherens junction, organism-specific biosystem; Adherens junction, conserved biosystem; Axon guidance, organism-specific biosystem; Developmental Biology, organism-specific biosystem; Herpes simplex infection, organism-specific biosystem; Herpes simplex infection, conserved biosystem;
Function: identical protein binding; kinase activity; metal ion binding; protein binding; protein domain specific binding; protein kinase regulator activity; protein kinase regulator activity; contributes_to protein serine/threonine kinase activity; receptor binding; transcription factor binding;

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