Recombinant Human CSHL1 293 Cell Lysate


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Recombinant Human CSHL1 293 Cell Lysate

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Cat.No. : CSHL1-7245HCL
Description : Antigen standard for chorionic somatomammotropin hormone-like 1 (CSHL1), transcript variant 3 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : CSHL1 chorionic somatomammotropin hormone-like 1 [ Homo sapiens ]
Official Symbol : CSHL1
Synonyms : CSHL1; chorionic somatomammotropin hormone-like 1; CSHP1; chorionic somatomammotropin CS 5; CS 5; CSL; hCS L; MGC149868; growth hormone cluster; chorionic somatomammotropin CS-5; CS-5; hCS-L;
Gene ID : 1444
mRNA Refseq : NM_001318
Protein Refseq : NP_001309
MIM : 603515
UniProt ID : Q14406
Chromosome Location : 17q22-q24
Function : hormone activity; metal ion binding;

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