Recombinant Human CSAG2 293 Cell Lysate


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Recombinant Human CSAG2 293 Cell Lysate

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Cat.No. : CSAG2-7251HCL
Description : Antigen standard for CSAG family, member 2 (CSAG2), transcript variant 2 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : CSAG2?CSAG family, member 2 [?Homo sapiens?(human) ]
Official Symbol : CSAG2
Synonyms : CSAG2; TRAG3; CSAG3B; CT24.2; TRAG-3; CSAG family, member 2; CSAG family, member 2; chondrosarcoma-associated gene 2/3 protein; CSAG family, member 3B; cancer/testis antigen 24.2; taxol resistance associated gene 3; cancer/testis antigen family 24, member 2; taxol-resistant-associated gene 3 protein; chondrosarcoma-associated gene 2/3 protein-like
Gene ID : 102723547
mRNA Refseq : XM_006724857
Protein Refseq : XP_006724920
Chromosome Location : Xq28

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