Recombinant Human CREM 293 Cell Lysate


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Recombinant Human CREM 293 Cell Lysate

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Cat.No. : CREM-7283HCL
Description : Antigen standard for cAMP responsive element modulator (CREM), transcript variant 6 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : CREM cAMP responsive element modulator [ Homo sapiens ]
Official Symbol : CREM
Synonyms : CREM; cAMP responsive element modulator; cAMP-responsive element modulator; hCREM 2; CREM 2beta-a protein; CREM 2alpha-b protein; cAMP response element modulator; inducible cAMP early repressor ICER; ICER; CREM-2; hCREM-2; MGC17881; MGC41893; MGC111110;
Gene ID : 1390
mRNA Refseq : NM_182719
Protein Refseq : NP_874388
MIM : 123812
UniProt ID : Q03060
Chromosome Location : 10p12.1-p11.1
Function : DNA binding; DNA binding; cAMP response element binding protein binding; protein binding; protein dimerization activity; sequence-specific DNA binding; sequence-specific DNA binding transcription factor activity;

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