Recombinant Human COX8C 293 Cell Lysate


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Recombinant Human COX8C 293 Cell Lysate

COX8C Related Products

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Cat.No. : COX8C-7322HCL
Description : Antigen standard for cytochrome c oxidase subunit 8C (COX8C), nuclear gene encoding mitochondrial protein is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : COX8C?cytochrome c oxidase subunit VIIIC [?Homo sapiens?(human) ]
Official Symbol : COX8C
Synonyms : COX8C; cytochrome c oxidase subunit VIIIC; COX8-3; cytochrome c oxidase subunit 8C, mitochondrial; COX VIII-3; cytochrome c oxidase subunit 8-3; cytochrome c oxidase subunit VIII; cytochrome c oxidase polypeptide 8; cytochrome c oxidase polypeptide VIII
Gene ID : 341947
mRNA Refseq : NM_182971
Protein Refseq : NP_892016
UniProt ID : Q7Z4L0
Chromosome Location : 14q32.12
Pathway : Alzheimer's disease, organism-specific biosystem; Cardiac muscle contraction, organism-specific biosystem; Cytochrome c oxidase, conserved biosystem; Huntington's disease, conserved biosystem
Function : cytochrome-c oxidase activity

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