"CLPTM1" Related Products

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Recombinant Human CLPTM1 293 Cell Lysate

Cat.No.: CLPTM1-7433HCL
Description: Antigen standard for cleft lip and palate associated transmembrane protein 1 (CLPTM1) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: CLPTM1 cleft lip and palate associated transmembrane protein 1 [ Homo sapiens ]
Official Symbol: CLPTM1
Synonyms: CLPTM1; cleft lip and palate associated transmembrane protein 1; cleft lip and palate transmembrane protein 1;
Gene ID: 1209
mRNA Refseq: NM_001294
Protein Refseq: NP_001285
MIM: 604783
UniProt ID: O96005
Chromosome Location: 19q13.3

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