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Recombinant Human CLCN2 293 Cell Lysate

Cat.No. : CLCN2-7475HCL
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  • Gene Information
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Description : Antigen standard for chloride channel 2 (CLCN2), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : CLCN2 chloride channel, voltage-sensitive 2 [ Homo sapiens ]
Official Symbol : CLCN2
Synonyms : CLCN2; chloride channel, voltage-sensitive 2; chloride channel 2; chloride channel protein 2; ClC 2; CLC2; EJM6; ECA2; ECA3; EGI3; EGMA; EJM8; CIC-2; EGI11; clC-2;
Gene ID : 1181
mRNA Refseq : NM_001171087
Protein Refseq : NP_001164558
MIM : 600570
UniProt ID : P51788
Chromosome Location : 3q26-qter
Pathway : Mineral absorption, organism-specific biosystem; Mineral absorption, conserved biosystem;
Function : voltage-gated chloride channel activity; voltage-gated ion channel activity;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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