"CHAF1A" Related Products

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Recombinant Human CHAF1A 293 Cell Lysate

Cat.No.: CHAF1A-7548HCL
Description: Antigen standard for chromatin assembly factor 1, subunit A (p150) (CHAF1A) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: CHAF1A chromatin assembly factor 1, subunit A (p150) [ Homo sapiens ]
Official Symbol: CHAF1A
Synonyms: CHAF1A; chromatin assembly factor 1, subunit A (p150); chromatin assembly factor 1 subunit A; CAF 1; CAF1; CAF1B; CAF1P150; chromatin assembly factor I (150 kDa); MGC71229; P150; hp150; CAF-I p150; CAF-1 subunit A; CAF-I 150 kDa subunit; chromatin assembly factor I p150 subunit; CAF-1;
Gene ID: 10036
mRNA Refseq: NM_005483
Protein Refseq: NP_005474
MIM: 601246
UniProt ID: Q13111
Chromosome Location: 19p13.3
Function: chromatin binding; chromo shadow domain binding; protein binding; unfolded protein binding;

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