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Recombinant Human CDIPT 293 Cell Lysate

Cat.No.: CDIPT-7634HCL
Description: Antigen standard for CDP-diacylglycerol--inositol 3-phosphatidyltransferase (phosphatidylinositol synthase) (CDIPT) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: CDIPT CDP-diacylglycerol--inositol 3-phosphatidyltransferase [ Homo sapiens ]
Official Symbol: CDIPT
Synonyms: CDIPT; CDP-diacylglycerol--inositol 3-phosphatidyltransferase; CDP diacylglycerol inositol 3 phosphatidyltransferase (phosphatidylinositol synthase); phosphatidylinositol synthase; PIS; PIS1; PI synthase; PtdIns synthase; MGC1328;
Gene ID: 10423
mRNA Refseq: NM_006319
Protein Refseq: NP_006310
MIM: 605893
UniProt ID: O14735
Chromosome Location: 16p12.1
Pathway: 3-phosphoinositide biosynthesis, organism-specific biosystem; 3-phosphoinositide biosynthesis, conserved biosystem; D-myo-inositol (1,4,5)-trisphosphate biosynthesis, organism-specific biosystem; D-myo-inositol (1,4,5)-trisphosphate biosynthesis, conserved biosystem; Glycerophospholipid metabolism, organism-specific biosystem; Glycerophospholipid metabolism, conserved biosystem; Inositol phosphate metabolism, organism-specific biosystem;
Function: CDP-diacylglycerol-inositol 3-phosphatidyltransferase activity; alcohol binding; diacylglycerol binding; manganese ion binding; phosphotransferase activity, for other substituted phosphate groups; sugar binding; transferase activity;

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