Recombinant Human CDH11 293 Cell Lysate

• Cat.No.: CDH11-7638HCL

Specification

• Description: Antigen standard for cadherin 11, type 2, OB-cadherin (osteoblast) (CDH11) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: CDH11 cadherin 11, type 2, OB-cadherin (osteoblast) [ Homo sapiens ]
• Official Symbol: CDH11
• Synonyms: CDH11; cadherin 11, type 2, OB-cadherin (osteoblast); cadherin-11; CAD11; OB; OB Cadherin; CDHOB; OSF-4
• Gene ID: 1009
• mRNA Refseq: NM_001797
• Protein Refseq: NP_001788
• MIM: 600023
• UniProt ID: P55287
• Chromosome Location: 16q21
• Pathway: Adherens junctions interactions, organism-specific biosystem; Cell junction organization, organism-specific biosystem; Cell-Cell communication, organism-specific biosystem; Cell-cell junction organization, organism-specific biosystem
• Function: calcium ion binding

Reviews

03/22/2023

The manufacturer's commitment to customer satisfaction is unmatched.

02/26/2023

Choosing the CDH11 protein from this manufacturer not only provides me with a superior product but also grants me access to unparalleled technical support.

07/24/2017

The manufacturer's commitment to customer satisfaction and their willingness to customize solutions based on specific experimental requirements is commendable.

Q&As

Are there any specific diseases or conditions associated with abnormal CDH11 expression?

Abnormal CDH11 expression is associated with conditions such as rheumatoid arthritis, osteoarthritis, and certain types of cancer.

Are there ongoing clinical trials targeting CDH11 for therapeutic purposes?

Yes, there are clinical trials exploring the inhibition of CDH11 as a potential treatment strategy for diseases like rheumatoid arthritis and certain types of cancer.

How is CDH11 implicated in osteoarthritis?

CDH11 is associated with osteoarthritis as it regulates the interaction between chondrocytes and synovial fibroblasts, contributing to the pathogenesis of the disease.

Where is CDH11 protein primarily expressed in the human body?

CDH11 is predominantly found in mesenchymal tissues, such as the bone and synovium, and is involved in cell-cell adhesion in these tissues.

How can CDH11 be utilized as a biomarker in clinical diagnostics?

CDH11 expression levels can be measured to assess the progression of certain diseases, such as osteoarthritis and certain cancers, providing valuable diagnostic information.