Recombinant Human CDC37L1 293 Cell Lysate


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Recombinant Human CDC37L1 293 Cell Lysate

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Cat.No. : CDC37L1-7659HCL
Description : Antigen standard for cell division cycle 37 homolog (S. cerevisiae)-like 1 (CDC37L1) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : CDC37L1 cell division cycle 37 homolog (S. cerevisiae)-like 1 [ Homo sapiens ]
Official Symbol : CDC37L1
Synonyms : CDC37L1; cell division cycle 37 homolog (S. cerevisiae)-like 1; CDC37 cell division cycle 37 homolog (S. cerevisiae) like 1 , cell division cycle 37 homolog (S. cerevisiae) like 1; hsp90 co-chaperone Cdc37-like 1; CDC37B; FLJ20639; HARC; Hsp90-associating relative of Cdc37; CDC37 cell division cycle 37 homolog-like 1; RP11-6J24.5;
Gene ID : 55664
mRNA Refseq : NM_017913
Protein Refseq : NP_060383
MIM : 610346
UniProt ID : Q7L3B6
Chromosome Location : 9p24.1

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