Recombinant Human CAV1 293 Cell Lysate

Cat.No.: CAV1-7822HCL
Description: Antigen standard for caveolin 1, caveolae protein, 22kDa (CAV1) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: CAV1 caveolin 1, caveolae protein, 22kDa [ Homo sapiens ]
Official Symbol: CAV1
Synonyms: CAV1; caveolin 1, caveolae protein, 22kDa; CAV, caveolin 1, caveolae protein, 22kD; caveolin-1; cell growth-inhibiting protein 32; CGL3; BSCL3; VIP21; MSTP085;
Gene ID: 857
mRNA Refseq: NM_001172895
Protein Refseq: NP_001166366
MIM: 601047
UniProt ID: Q03135
Chromosome Location: 7q31
Pathway: ALK1 signaling events, organism-specific biosystem; Androgen Receptor Signaling Pathway, organism-specific biosystem; Bacterial invasion of epithelial cells, organism-specific biosystem; Bacterial invasion of epithelial cells, conserved biosystem; Basigin interactions, organism-specific biosystem; Canonical Wnt signaling pathway, organism-specific biosystem; Cell surface interactions at the vascular wall, organism-specific biosystem;
Function: cholesterol binding; kinase binding; nitric-oxide synthase binding; patched binding; peptidase activator activity; protein binding; protein complex scaffold; receptor binding; structural molecule activity; syntaxin binding;

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