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Recombinant Human CAMK2G 293 Cell Lysate

Cat.No.: CAMK2G-7876HCL
Description: Antigen standard for calcium/calmodulin-dependent protein kinase II gamma (CAMK2G), transcript variant 2 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: CAMK2G calcium/calmodulin-dependent protein kinase II gamma [ Homo sapiens ]
Official Symbol: CAMK2G
Synonyms: CAMK2G; calcium/calmodulin-dependent protein kinase II gamma; calcium/calmodulin dependent protein kinase (CaM kinase) II gamma , CAMKG; calcium/calmodulin-dependent protein kinase type II subunit gamma; caMK-II subunit gamma; calcium/calmodulin-dependent protein kinase (CaM kinase) II gamma; CAMK; CAMKG; CAMK-II; FLJ16043; MGC26678;
Gene ID: 818
mRNA Refseq: NM_172169
Protein Refseq: NP_751909
MIM: 602123
UniProt ID: Q13555
Chromosome Location: 10q22
Pathway: Activation of NMDA receptor upon glutamate binding and postsynaptic events, organism-specific biosystem; Amphetamine addiction, organism-specific biosystem; Amphetamine addiction, conserved biosystem; BCR signaling pathway, organism-specific biosystem; CREB phosphorylation through the activation of CaMKII, organism-specific biosystem; CREB phosphorylation through the activation of Ras, organism-specific biosystem; Calcium Regulation in the Cardiac Cell, organism-specific biosystem;
Function: ATP binding; ATP binding; calcium-dependent protein serine/threonine phosphatase activity; calmodulin binding; calmodulin binding; calmodulin-dependent protein kinase activity; calmodulin-dependent protein kinase activity; nucleotide binding;

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