Recombinant Human CALHM1 293 Cell Lysate

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Recombinant Human CALHM1 293 Cell Lysate

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Cat.No. : CALHM1-7892HCL
Description : Antigen standard for calcium homeostasis modulator 1 (CALHM1) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : CALHM1 calcium homeostasis modulator 1 [ Homo sapiens ]
Official Symbol : CALHM1
Synonyms : CALHM1; calcium homeostasis modulator 1; FAM26C, family with sequence similarity 26, member C; calcium homeostasis modulator protein 1; family with sequence similarity 26, member C; FAM26C; MGC39514; MGC39617;
Gene ID : 255022
mRNA Refseq : NM_001001412
Protein Refseq : NP_001001412
MIM : 612234
UniProt ID : Q8IU99
Chromosome Location : 10q24.33
Function : calcium channel activity; identical protein binding; ion channel activity;

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