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Recombinant Human CAB39 293 Cell Lysate

Cat.No.: CAB39-7912HCL
Description: Antigen standard for calcium binding protein 39 (CAB39), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: CAB39 calcium binding protein 39 [ Homo sapiens ]
Official Symbol: CAB39
Synonyms: CAB39; calcium binding protein 39; calcium-binding protein 39; CGI 66; MO25; MO25alpha; CGI-66; FLJ22682;
Gene ID: 51719
mRNA Refseq: NM_001130849
Protein Refseq: NP_001124321
MIM: 612174
UniProt ID: Q9Y376
Chromosome Location: 2q37.1
Pathway: AMPK signaling, organism-specific biosystem; Endochondral Ossification, organism-specific biosystem; Energy dependent regulation of mTOR by LKB1-AMPK, organism-specific biosystem; IRS-mediated signalling, organism-specific biosystem; IRS-related events, organism-specific biosystem; Insulin receptor signalling cascade, organism-specific biosystem; LKB1 signaling events, organism-specific biosystem;
Function: kinase binding; protein binding; protein kinase activator activity; contributes_to protein serine/threonine kinase activity; protein serine/threonine kinase activity;

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