Recombinant Human C9orf24 293 Cell Lysate

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Recombinant Human C9orf24 293 Cell Lysate

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Cat.No. : C9orf24-7935HCL
Description : Antigen standard for chromosome 9 open reading frame 24 (C9orf24), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : C9orf24?chromosome 9 open reading frame 24 [?Homo sapiens?(human) ]
Official Symbol : C9orf24
Synonyms : C9orf24; chromosome 9 open reading frame 24; CBE1; SMRP1; NYD-SP22; bA573M23.4; spermatid-specific manchette-related protein 1; ciliated bronchial epithelium 1; testis development protein NYD-SP22; ciliated bronchial epithelial protein 1
Gene ID : 84688
mRNA Refseq : NM_032596
Protein Refseq : NP_115985
UniProt ID : Q8NCR6
Chromosome Location : 9p13.3
Function : alpha-tubulin binding

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◆ Recombinant Protein

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