"C3orf75" Related Products

Recombinant Human C3orf75 293 Cell Lysate

Cat.No.: C3orf75-8038HCL
Description: Antigen standard for chromosome 3 open reading frame 75 (C3orf75) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: C3orf75 chromosome 3 open reading frame 75 [ Homo sapiens ]
Official Symbol: C3orf75
Synonyms: C3ORF75; chromosome 3 open reading frame 75; TMEM103, transmembrane protein 103; elongator complex protein 6 homolog; FLJ20211; UPF0405 protein C3orf75; transmembrane protein 103; angiotonin-transactivated protein 1; TMEM103;
Gene ID: 54859
mRNA Refseq: NM_001031703
Protein Refseq: NP_001026873
UniProt ID: Q0PNE2
Chromosome Location: 3p21.31
Function: molecular_function;

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