"COA7" Related Products

Recombinant Human C1orf163 293 Cell Lysate

Cat.No.: COA7-8173HCL
Description: Antigen standard for chromosome 1 open reading frame 163 (C1orf163) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: COA7 cytochrome c oxidase assembly factor 7 (putative) [ Homo sapiens ]
Official Symbol: COA7
Synonyms: RESA1; SELRC1; C1orf163; cytochrome c oxidase assembly factor 7; Sel1 repeat containing 1; respiratory chain assembly 1; beta-lactamase hcp-like protein; sel1 repeat-containing protein 1; respiratory chain assembly factor 1; hcp beta-lactamase-like protein C1orf163
Gene ID: 65260
mRNA Refseq: NM_023077
Protein Refseq: NP_075565
MIM: 615623
UniProt ID: Q96BR5
Chromosome Location: 1p32.3
Products Types ◆ Recombinant Protein

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