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Recombinant Human C17orf80 293 Cell Lysate

Cat.No. : C17orf80-8228HCL
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  • Gene Information
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Description : Antigen standard for chromosome 17 open reading frame 80 (C17orf80), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : C17orf80 chromosome 17 open reading frame 80 [ Homo sapiens ]
Official Symbol : C17orf80
Synonyms : C17ORF80; chromosome 17 open reading frame 80; uncharacterized protein C17orf80; FLJ20721; HLC 8; MIG3; migration inducing protein 3; SPEP1; sperm expressed protein 1; sperm-expressed protein 1; migration-inducing protein 3; lung cancer-related protein 8; human lung cancer oncogene 8 protein; cell migration-inducing gene 3 protein; HLC-8;
Gene ID : 55028
mRNA Refseq : NM_001100621
Protein Refseq : NP_001094091
UniProt ID : Q9BSJ5
Chromosome Location : 17q25.1

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