"C17orf28" Related Products

Recombinant Human C17orf28 293 Cell Lysate

Cat.No.: C17orf28-8240HCL
Description: Antigen standard for chromosome 17 open reading frame 28 (C17orf28) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: C17orf28 chromosome 17 open reading frame 28 [ Homo sapiens ]
Official Symbol: C17orf28
Synonyms: C17ORF28; chromosome 17 open reading frame 28; protein hid-1 homolog; DMC1; downregulated in multiple cancer 1; down-regulated in multiple cancers 1; UPF0663 transmembrane protein C17orf28; FLJ43526;
Gene ID: 283987
mRNA Refseq: NM_030630
Protein Refseq: NP_085133
MIM: 605752
UniProt ID: Q8IV36
Chromosome Location: 17q25.1

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