Recombinant Human C15orf41 293 Cell Lysate

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Recombinant Human C15orf41 293 Cell Lysate

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Cat.No. : C15orf41-8265HCL
Description : Antigen standard for chromosome 15 open reading frame 41 (C15orf41), transcript variant 2 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : C15orf41 chromosome 15 open reading frame 41 [ Homo sapiens ]
Official Symbol : C15orf41
Synonyms : C15ORF41; chromosome 15 open reading frame 41; uncharacterized protein C15orf41; FLJ22851; HH114; MGC11326;
Gene ID : 84529
mRNA Refseq : NM_001130010
Protein Refseq : NP_001123482
UniProt ID : Q9Y2V0
Chromosome Location : 15q14

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