"IFT43" Related Products

Recombinant Human C14orf179 293 Cell Lysate

Cat.No.: IFT43-8278HCL
Description: Antigen standard for chromosome 14 open reading frame 179 (C14orf179), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: IFT43 intraflagellar transport 43 [ Homo sapiens ]
Official Symbol: IFT43
Synonyms: CED3; C14orf179; intraflagellar transport protein 43 homolog; IFT complex A subunit; intraflagellar transport 43 homolog
Gene ID: 112752
mRNA Refseq: NM_001102564
Protein Refseq: NP_001096034
MIM: 614068
UniProt ID: Q96FT9
Chromosome Location: 14q24.3

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