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Recombinant Human BRE 293 Cell Lysate

Cat.No.: BRE-8409HCL
Description: Antigen standard for brain and reproductive organ-expressed (TNFRSF1A modulator) (BRE), transcript variant 3 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: BRE brain and reproductive organ-expressed (TNFRSF1A modulator) [ Homo sapiens ]
Official Symbol: BRE
Synonyms: BRE; brain and reproductive organ-expressed (TNFRSF1A modulator); BRCA1-A complex subunit BRE; BRCA1/BRCA2 containing complex; subunit 4; BRCC4; BRCC45; BRCA1/BRCA2-containing complex subunit 45; BRCA1/BRCA2-containing complex, subunit 4; brain and reproductive organ-expressed protein;
Gene ID: 9577
mRNA Refseq: NM_004899
Protein Refseq: NP_004890
MIM: 610497
UniProt ID: Q9NXR7
Chromosome Location: 2p23
Function: peroxisome targeting sequence binding; polyubiquitin binding; protein binding; tumor necrosis factor receptor binding;

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