Recombinant Human BMX 293 Cell Lysate

• Cat.No.: BMX-8427HCL

Specification

• Description: Antigen standard for BMX non-receptor tyrosine kinase (BMX), transcript variant 2 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: BMX BMX non-receptor tyrosine kinase [ Homo sapiens ]
• Official Symbol: BMX
• Synonyms: BMX; BMX non-receptor tyrosine kinase; cytoplasmic tyrosine-protein kinase BMX; ETK; PSCTK3; NTK38 tyrosine kinase; Etk/Bmx cytosolic tyrosine kinase; epithelial and endothelial tyrosine kinase; bone marrow tyrosine kinase gene in chromosome X protein; PSCTK2
• Gene ID: 660
• mRNA Refseq: NM_001721
• Protein Refseq: NP_001712
• MIM: 300101
• UniProt ID: P51813
• Chromosome Location: Xp22.2
• Pathway: Angiopoietin receptor Tie2-mediated signaling, organism-specific biosystem; Apoptosis, organism-specific biosystem; Apoptotic cleavage of cellular proteins, organism-specific biosystem; Apoptotic executionphase, organism-specific biosystem; IL-3 Signaling Pathway, organism-specific biosystem; IL-6 Signaling Pathway, organism-specific biosystem; Signaling events mediated by focal adhesion kinase, organism-specific biosystem
• Function: ATP binding; metal ion binding; non-membrane spanning protein tyrosine kinase activity; nucleotide binding; protein binding; protein tyrosine kinase activity; signal transducer activity

Reviews

02/22/2022

Accurate mass spectrometry analysis; essential for proteomics research.

10/02/2021

Reliable western blot results; a staple for our lab work.

10/15/2018

Precise protein quantification; vital for our research projects.

Q&As

How does BMX interact with other members of the Tec family kinases in immune cell signaling?

BMX interacts with Tec family kinases to regulate immune cell signaling, affecting immune responses.

What are the effects of BMX inhibition on the angiogenesis process in tumor models?

Inhibition of BMX reduces angiogenesis in tumor models, suggesting a role in tumor growth and metastasis.

What are the potential therapeutic applications of targeting BMX in cardiovascular diseases?

Targeting BMX in cardiovascular diseases offers potential therapeutic benefits, possibly impacting vascular remodeling and function.

What are the specific substrates of BMX kinase in different cell types and how do they influence cell signaling pathways?

BMX kinase substrates vary by cell type, impacting diverse signaling pathways related to cell growth and survival.

How does the expression of BMX vary in normal versus cancerous tissues, particularly in breast and prostate cancers?

BMX expression differs in cancerous tissues, with heightened levels in certain breast and prostate cancers, indicating a potential role in tumor progression.

What role does BMX play in the regulation of inflammatory responses in autoimmune diseases?

BMX is involved in regulating inflammatory responses, playing a significant role in autoimmune disease mechanisms.

How does phosphorylation affect the activity and stability of BMX in cellular contexts?

Phosphorylation alters BMX activity and stability, influencing its function in cellular signaling processes.