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Recombinant Human BLOC1S1 293 Cell Lysate

Cat.No.: BLOC1S1-8444HCL
Description: Antigen standard for biogenesis of lysosomal organelles complex-1, subunit 1 (BLOC1S1) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: BLOC1S1 biogenesis of lysosomal organelles complex-1, subunit 1 [ Homo sapiens ]
Official Symbol: BLOC1S1
Synonyms: RT14; BLOS1; MICoA; GCN5L1
Gene ID: 2647
mRNA Refseq: NM_001487.3
Protein Refseq: NP_001478.2
MIM: 601444
UniProt ID: P78537
Chromosome Location: 12q13-q14
Pathway: Clathrin derived vesicle budding, organism-specific biosystem;Golgi Associated Vesicle Biogenesis, organism-specific biosystem;Lysosome Vesicle Biogenesis, organism-specific biosystem;Membrane Trafficking, organism-specific biosystem;trans-Golgi Network Vesicle Budding, organism-specific biosystem;
Function: protein binding;

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