Recombinant Human BHLHE40 293 Cell Lysate

• Cat.No.: BHLHE40-8459HCL

Specification

• Description: Antigen standard for basic helix-loop-helix family, member e40 (BHLHE40) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: BHLHE40 basic helix-loop-helix family, member e40 [ Homo sapiens ]
• Official Symbol: BHLHE40
• Synonyms: BHLHE40; basic helix-loop-helix family, member e40; basic helix loop helix domain containing, class B, 2 , BHLHB2, STRA13; class E basic helix-loop-helix protein 40; differentiated embryo chondrocyte expressed gene 1; bHLHe40; DEC1; differentially expressed in chondrocytes 1; class B basic helix-loop-helix protein 2; stimulated by retinoic acid gene 13 protein; enhancer-of-split and hairy-related protein 2; differentially expressed in chondrocytes protein 1; basic helix-loop-helix domain containing, class B, 2; HLHB2; BHLHB2; STRA13; Stra14; SHARP-2; FLJ99214
• Gene ID: 8553
• mRNA Refseq: NM_003670
• Protein Refseq: NP_003661
• MIM: 604256
• UniProt ID: O14503
• Chromosome Location: 3p26
• Pathway: BMAL1:CLOCK/NPAS2 Activates Circadian Expression, organism-specific biosystem; Circadian Clock, organism-specific biosystem; Circadian rhythm - mammal, organism-specific biosystem; Circadian rhythm - mammal, conserved biosystem; Circadian rhythm pathway, organism-specific biosystem; HIF-1-alpha transcription factor network, organism-specific biosystem; HIF-2-alpha transcription factor network, organism-specific biosystem
• Function: DNA binding; E-box binding; MRF binding; RNA polymerase II activating transcription factor binding; RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription; RNA polymerase II transcription factor binding transcription factor activity involved in negative regulation of transcription; bHLH transcription factor binding; protein binding; protein domain specific binding; protein heterodimerization activity; protein homodimerization activity; sequence-specific DNA binding RNA polymerase II transcription factor activity; sequence-specific DNA binding transcription factor activity; sequence-specific distal enhancer binding RNA polymerase II transcription factor activity