"BDH2" Related Products

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Recombinant Human BDH2 293 Cell Lysate

Cat.No.: BDH2-8472HCL
Description: Antigen standard for 3-hydroxybutyrate dehydrogenase, type 2 (BDH2) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: BDH2 3-hydroxybutyrate dehydrogenase, type 2 [ Homo sapiens ]
Official Symbol: BDH2
Synonyms: BDH2; 3-hydroxybutyrate dehydrogenase, type 2; dehydrogenase/reductase (SDR family) member 6 , DHRS6; 3-hydroxybutyrate dehydrogenase type 2; FLJ13261; PRO20933; SDR15C1; short chain dehydrogenase/reductase family 15C; member 1; UCPA OR; UNQ6308; oxidoreductase UCPA; R-beta-hydroxybutyrate dehydrogenase; dehydrogenase/reductase SDR family member 6; dehydrogenase/reductase (SDR family) member 6; short chain dehydrogenase/reductase family 15C, member 1; DHRS6; EFA6R; UCPA-OR;
Gene ID: 56898
mRNA Refseq: NM_020139
Protein Refseq: NP_064524
UniProt ID: Q9BUT1
Chromosome Location: 4q24
Pathway: Butanoate metabolism, organism-specific biosystem; Butanoate metabolism, conserved biosystem; Metabolic pathways, organism-specific biosystem; Synthesis and degradation of ketone bodies, organism-specific biosystem; Synthesis and degradation of ketone bodies, conserved biosystem; ketolysis, organism-specific biosystem; ketolysis, conserved biosystem;
Function: 3-hydroxybutyrate dehydrogenase activity; NAD binding; oxidoreductase activity; oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor;

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