"BCAT1" Related Products

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Recombinant Human BCAT1 293 Cell Lysate

Cat.No.: BCAT1-8495HCL
Description: Antigen standard for branched chain aminotransferase 1, cytosolic (BCAT1) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: BCAT1 branched chain amino-acid transaminase 1, cytosolic [ Homo sapiens ]
Official Symbol: BCAT1
Synonyms: BCAT1; branched chain amino-acid transaminase 1, cytosolic; BCT1, branched chain aminotransferase 1, cytosolic; branched-chain-amino-acid aminotransferase, cytosolic; placental protein 18; branched chain aminotransferase 1, cytosolic; BCT1; PP18; BCATC; ECA39; MECA39; PNAS121; DKFZp686E12175;
Gene ID: 586
mRNA Refseq: NM_001178091
Protein Refseq: NP_001171562
MIM: 113520
UniProt ID: P54687
Chromosome Location: 12pter-q12
Pathway: Branched-chain amino acid catabolism, organism-specific biosystem; Leucine degradation, leucine =>acetoacetate + acetyl-CoA, organism-specific biosystem; Leucine degradation, leucine => acetoacetate + acetyl-CoA, conserved biosystem; Metabolic pathways, organism-specific biosystem; Metabolism, organism-specific biosystem;
Function: L-isoleucine transaminase activity; L-leucine transaminase activity; L-valine transaminase activity; branched-chain-amino-acid transaminase activity; transferase activity;

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