Recombinant Human AXIN2 293 Cell Lysate

• Cat.No.: AXIN2-8554HCL

Specification

• Description: Antigen standard for axin 2 (AXIN2) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid (True ORF cDNA clone RC210931, OriGene Technologies, Inc.) and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: AXIN2 axin 2 [ Homo sapiens ]
• Official Symbol: AXIN2
• Synonyms: AXIN2; axin 2; axin-2; axil; conductin; DKFZp781B0869; MGC126582; axin-like protein; axis inhibition protein 2; AXIL; MGC10366
• Gene ID: 8313
• mRNA Refseq: NM_004655
• Protein Refseq: NP_004646
• MIM: 604025
• UniProt ID: Q9Y2T1
• Chromosome Location: 17q23-q24
• Pathway: Basal cell carcinoma, organism-specific biosystem; Basal cell carcinoma, conserved biosystem; Canonical Wnt signaling pathway, organism-specific biosystem; Colorectal cancer, organism-specific biosystem; Colorectal cancer, conserved biosystem; Endometrial cancer, organism-specific biosystem; Endometrial cancer, conserved biosystem
• Function: GTPase activator activity; I-SMAD binding; armadillo repeat domain binding; beta-catenin binding; beta-catenin binding; enzyme binding; protein kinase binding; signal transducer activity; ubiquitin protein ligase binding