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Recombinant Human AURKA 293 Cell Lysate

Cat.No. : AURKA-8564HCL
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  • Gene Information
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Description : Antigen standard for aurora kinase A (AURKA), transcript variant 4 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : AURKA aurora kinase A [ Homo sapiens ]
Official Symbol : AURKA
Synonyms : AURKA; aurora kinase A; serine/threonine kinase 6 , serine/threonine kinase 15 , STK6, STK15; ARK1; AurA; BTAK; PPP1R47; protein phosphatase 1; regulatory subunit 47; STK7; ARK-1; hARK1; aurora 2; IPL1-related kinase; aurora-related kinase 1; aurora/IPL1-like kinase; serine/threonine kinase 6; aurora/IPL1-related kinase 1; breast tumor-amplified kinase; breast-tumor-amplified kinase; serine/threonine-protein kinase 6; serine/threonine protein kinase 15; serine/threonine-protein kinase 15; serine/threonine-protein kinase aurora-A; protein phosphatase 1, regulatory subunit 47; AIK; AURA; STK6; STK15; AURORA2; MGC34538;
Gene ID : 6790
mRNA Refseq : NM_198435
Protein Refseq : NP_940837
MIM : 603072
UniProt ID : O14965
Chromosome Location : 20q13
Pathway : APC/C-mediated degradation of cell cycle proteins, organism-specific biosystem; APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1, organism-specific biosystem; Aurora A signaling, organism-specific biosystem; Aurora B signaling, organism-specific biosystem; Cell Cycle, organism-specific biosystem; Cell Cycle, Mitotic, organism-specific biosystem; Oocyte meiosis, organism-specific biosystem;
Function : ATP binding; kinase activity; nucleotide binding; protein binding; protein kinase activity; protein kinase binding; protein serine/threonine kinase activity; protein serine/threonine/tyrosine kinase activity; ubiquitin protein ligase binding;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (6)

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How does AURKA interact with cell cycle regulators? 07/19/2022

AURKA protein interacts with a variety of cell cycle regulatory proteins, including Cdk1, Plk1, TPX2, etc., to regulate the progression of cell cycle.

What is the role of AURKA protein in the cell cycle? 09/26/2020

The protein plays an important role in the G2 and M phases of the cell cycle and is involved in the regulation of nuclear division, chromosome segregation, and cell mitosis.

Is AURKA protein involved in apoptosis and DNA damage repair? 09/09/2020

AURKA protein may be involved in the regulation of apoptosis and DNA damage repair, but the specific mechanism needs to be further studied.

What is the regulatory mechanism of AURKA protein activity? 01/02/2020

The activity is regulated by a variety of factors, including phosphorylation modification, protein interaction and cell cycle regulation.

Where are the cells of AURKA protein? 10/06/2019

It is mainly localized in the cytoplasm and nucleus and is involved in cell cycle regulation and cell division processes.

What is the role of AURKA protein in tumorigenesis? 01/01/2019

This protein plays an important role in tumorigenesis, and its overexpression or mutation may lead to abnormal cell proliferation, chromosome instability and tumorigenesis.

Customer Reviews (3)

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Reviews
12/08/2022

    AURKA's exceptional stability allows for its reliable use in a range of applications, making it a top choice for researchers.

    02/10/2022

      This protein's exceptional activity allows for its reliable use in a range of applications, making it a top choice for researchers.

      10/24/2020

        Due to the extensive storage conditions of proteins, the experimental process is more stable and reliable, which provides a strong guarantee for scientific research.

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