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Recombinant Human ATP6V1E1 293 Cell Lysate

Cat.No.: ATP6V1E1-8580HCL
Description: Antigen standard for ATPase, H+ transporting, lysosomal 31kDa, V1 subunit E1 (ATP6V1E1), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: ATP6V1E1 ATPase, H+ transporting, lysosomal 31kDa, V1 subunit E1 [ Homo sapiens ]
Official Symbol: ATP6V1E1
Synonyms: ATP6V1E1; ATPase, H+ transporting, lysosomal 31kDa, V1 subunit E1; ATP6E, ATP6V1E, ATPase, H+ transporting, lysosomal (vacuolar proton pump) 31kD , ATPase, H+ transporting, lysosomal 31kDa, V1 subunit E isoform 1; V-type proton ATPase subunit E 1; ATP6E2; P31; Vma4; V-ATPase, subunit E; V-ATPase subunit E 1; V-ATPase 31 kDa subunit; vacuolar proton pump subunit E 1; H+-transporting ATP synthase chain E, vacuolar; H(+)-transporting two-sector ATPase, 31kDa subunit; ATP6E; ATP6V1E;
Gene ID: 529
mRNA Refseq: NM_001039366
Protein Refseq: NP_001034455
MIM: 108746
UniProt ID: P36543
Chromosome Location: 22q11.2
Pathway: Collecting duct acid secretion, organism-specific biosystem; Collecting duct acid secretion, conserved biosystem; Epithelial cell signaling in Helicobacter pylori infection, organism-specific biosystem; Epithelial cell signaling in Helicobacter pylori infection, conserved biosystem; Insulin receptor recycling, organism-specific biosystem; Iron uptake and transport, organism-specific biosystem; Metabolic pathways, organism-specific biosystem;
Function: hydrogen-exporting ATPase activity, phosphorylative mechanism; hydrolase activity; protein binding; proton-transporting ATPase activity, rotational mechanism;

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