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Recombinant Human ATP6V0D2 293 Cell Lysate

Cat.No. : ATP6V0D2-8587HCL
  • Specification
  • Gene Information
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Description : Antigen standard for ATPase, H+ transporting, lysosomal 38kDa, V0 subunit d2 (ATP6V0D2) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : ATP6V0D2 ATPase, H+ transporting, lysosomal 38kDa, V0 subunit d2 [ Homo sapiens ]
Official Symbol : ATP6V0D2
Synonyms : ATP6V0D2; ATPase, H+ transporting, lysosomal 38kDa, V0 subunit d2; ATPase, H+ transporting, lysosomal 38kD, V0 subunit d isoform 2 , ATPase, H+ transporting, lysosomal 38kDa, V0 subunit d isoform 2 , ATPase, H+ transporting, lysosomal 38kDa, V0 subunit D2; V-type proton ATPase subunit d 2; ATP6D2; FLJ38708; VMA6; V-ATPase subunit d 2; vacuolar proton pump subunit d 2;
Gene ID : 245972
mRNA Refseq : NM_152565
Protein Refseq : NP_689778
UniProt ID : Q8N8Y2
Chromosome Location : 8q21.3
Pathway : Collecting duct acid secretion, organism-specific biosystem; Collecting duct acid secretion, conserved biosystem; Epithelial cell signaling in Helicobacter pylori infection, organism-specific biosystem; Epithelial cell signaling in Helicobacter pylori infection, conserved biosystem; Insulin receptor recycling, organism-specific biosystem; Iron uptake and transport, organism-specific biosystem; Lysosome, organism-specific biosystem;
Function : hydrogen ion transmembrane transporter activity; protein binding;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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