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Recombinant Human ART5 293 Cell Lysate

Cat.No. : ART5-8671HCL
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  • Gene Information
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Description : Antigen standard for ADP-ribosyltransferase 5 (ART5), transcript variant 2 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : ART5 ADP-ribosyltransferase 5 [ Homo sapiens ]
Official Symbol : ART5
Synonyms : ART5; ADP-ribosyltransferase 5; ecto-ADP-ribosyltransferase 5; mono(ADP-ribosyl)transferase 5; NAD(P)(+)--arginine ADP-ribosyltransferase 5; MGC22848;
Gene ID : 116969
mRNA Refseq : NM_001079536
Protein Refseq : NP_001073004
MIM : 610625
UniProt ID : Q96L15
Chromosome Location : 11p15.4
Function : NAD(P)+-protein-arginine ADP-ribosyltransferase activity; NAD+ ADP-ribosyltransferase activity; NAD+ nucleosidase activity; transferase activity, transferring glycosyl groups;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (7)

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How does the ADP-ribosylation catalyzed by ART5 affect target proteins? 11/01/2022

The ADP-ribosylation catalyzed by ART5 can modify target proteins, potentially influencing their functions or interactions with other molecules.

Does ART5 play a role in fertility or reproductive processes? 02/27/2021

Given its expression in the testis and ovaries, ART5 likely plays a role in fertility or reproductive processes. However, the specific mechanisms are not yet fully understood.

Can ART5 activity be modulated by external stimuli or factors? 11/20/2020

The modulation of ART5 activity by external stimuli or factors is not well characterized. Further studies are needed to elucidate potential regulators.

Does ART5 have any known biological substrates? 08/26/2020

While the specific substrates of ART5 are yet to be fully identified, it may modify certain proteins involved in reproductive processes.

Are there any known ART5 interacting proteins? 04/20/2020

The specific proteins that interact with ART5 have not been thoroughly identified. It requires further research to determine its binding partners.

Is there any evidence linking ART5 to cancer development or progression? 08/29/2018

The potential association between ART5 and cancer development or progression remains unknown. More research is needed to explore this possibility.

Is ART5 involved in any signaling pathways or protein networks? 06/27/2017

The involvement of ART5 in signaling pathways or protein networks is not well established. Further research is needed to determine its potential interactions.

Customer Reviews (3)

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Reviews
08/11/2020

    Its outstanding performance, coupled with its ease of use, has enhanced the overall efficiency and accuracy of my research.

    04/26/2019

      Its ability to maintain structural integrity and stability ensures accurate imaging, enabling me to gain deeper insights into the three-dimensional organization and interactions of the proteins under investigation.

      03/04/2017

        The ART5 Protein has greatly contributed to the success of my experiments, providing reliable and robust data.

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