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Recombinant Human ARPC4 293 Cell Lysate

Cat.No. : ARPC4-8684HCL
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  • Gene Information
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Description : Antigen standard for actin related protein 2/3 complex, subunit 4, 20kDa (ARPC4), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : ARPC4 actin related protein 2/3 complex, subunit 4, 20kDa [ Homo sapiens ]
Official Symbol : ARPC4
Synonyms : ARPC4; actin related protein 2/3 complex, subunit 4, 20kDa; actin related protein 2/3 complex, subunit 4 (20 kD); actin-related protein 2/3 complex subunit 4; actin related protein 2/3 complex; subunit 4 (20 kD); ARC20; Arp2/3 protein complex subunit p20; p20 Arc; arp2/3 complex 20 kDa subunit; arp2/3 protein complex subunit p20; P20-ARC; MGC13544;
Gene ID : 10093
mRNA Refseq : NM_001024959
Protein Refseq : NP_001020130
MIM : 604226
UniProt ID : P59998
Chromosome Location : 3p25
Pathway : B Cell Receptor Signaling Pathway, organism-specific biosystem; Bacterial invasion of epithelial cells, organism-specific biosystem; Bacterial invasion of epithelial cells, conserved biosystem; CDC42 signaling events, organism-specific biosystem; ErbB1 downstream signaling, organism-specific biosystem; Fc gamma R-mediated phagocytosis, organism-specific biosystem; Fc gamma R-mediated phagocytosis, conserved biosystem;
Function : contributes_to actin filament binding; protein binding; contributes_to protein binding, bridging;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (7)

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Can ARPC4 expression be regulated by extracellular signals? 08/27/2022

Yes, ARPC4 expression can be influenced by extracellular signals. Various signaling pathways, such as the Rho GTPase pathway and growth factor signaling, can activate or inhibit the expression of genes encoding ARPC4 and other subunits of the ARP2/3 complex, thereby modulating actin cytoskeleton dynamics.

Are there any known drugs or compounds that can modulate ARPC4 activity? 02/17/2021

At present, there are no specific drugs or compounds known to directly modulate ARPC4 activity. However, targeting actin cytoskeleton dynamics and actin regulatory proteins that indirectly affect ARPC4 function has shown potential in preclinical studies as therapeutic approaches for various diseases.

Are there any diseases associated with mutations in the ARPC4 gene? 01/05/2018

Mutations in the ARPC4 gene have not been directly linked to any specific diseases. However, dysregulation of the ARP2/3 complex, including ARPC4, has been implicated in several pathological conditions such as cancer metastasis, neurological disorders, and immune system dysfunction.

Is ARPC4 expression specific to certain cell types or tissues? 09/24/2017

ARPC4 is considered a ubiquitously expressed protein, meaning it is expressed in a wide range of tissues and cell types throughout the body. Its expression is necessary for essential cellular processes involving actin cytoskeleton dynamics.

Are there any known interacting proteins with ARPC4? 01/09/2017

Yes, ARPC4 interacts with other subunits of the ARP2/3 complex, including ARPC1, ARPC2, ARPC3, ARPC5, and ARPC6. Additionally, ARPC4 can interact with actin-binding proteins, such as cortactin and coronin, which regulate actin filament assembly and stability.

Can changes in ARPC4 expression levels affect cell migration? 08/05/2016

Yes, alterations in ARPC4 expression levels can impact cell migration. The ARP2/3 complex, including ARPC4, is essential for actin filament nucleation and branching, which are crucial for cell motility. Changes in ARPC4 expression can disrupt proper actin cytoskeletal dynamics, impairing cell migration.

Can changes in ARPC4 expression affect cell division? 05/06/2016

Yes, changes in ARPC4 expression can impact cell division. The ARP2/3 complex, including ARPC4, is involved in actin-dependent processes during cell division, such as cytokinesis and chromosome segregation. Disruption of ARPC4 expression can lead to defects in these cellular processes.

Customer Reviews (3)

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Reviews
01/15/2021

    Renowned for its reliability and consistency, this protein offers researchers the confidence to achieve accurate and reproducible results in their studies.

    06/15/2017

      One notable advantage of the ARPC4 protein is the excellent technical support provided by its manufacturer.

      02/17/2016

        The ARPC4 protein is known for its exceptional quality, making it a highly suitable choice for meeting diverse experimental needs.

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