"ARPC4" Related Products

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Recombinant Human ARPC4 293 Cell Lysate

Cat.No.: ARPC4-8684HCL
Description: Antigen standard for actin related protein 2/3 complex, subunit 4, 20kDa (ARPC4), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: ARPC4 actin related protein 2/3 complex, subunit 4, 20kDa [ Homo sapiens ]
Official Symbol: ARPC4
Synonyms: ARPC4; actin related protein 2/3 complex, subunit 4, 20kDa; actin related protein 2/3 complex, subunit 4 (20 kD); actin-related protein 2/3 complex subunit 4; actin related protein 2/3 complex; subunit 4 (20 kD); ARC20; Arp2/3 protein complex subunit p20; p20 Arc; arp2/3 complex 20 kDa subunit; arp2/3 protein complex subunit p20; P20-ARC; MGC13544;
Gene ID: 10093
mRNA Refseq: NM_001024959
Protein Refseq: NP_001020130
MIM: 604226
UniProt ID: P59998
Chromosome Location: 3p25
Pathway: B Cell Receptor Signaling Pathway, organism-specific biosystem; Bacterial invasion of epithelial cells, organism-specific biosystem; Bacterial invasion of epithelial cells, conserved biosystem; CDC42 signaling events, organism-specific biosystem; ErbB1 downstream signaling, organism-specific biosystem; Fc gamma R-mediated phagocytosis, organism-specific biosystem; Fc gamma R-mediated phagocytosis, conserved biosystem;
Function: contributes_to actin filament binding; protein binding; contributes_to protein binding, bridging;

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