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Recombinant Human ARNTL 293 Cell Lysate

Cat.No.: ARNTL-8690HCL
Description: Antigen standard for aryl hydrocarbon receptor nuclear translocator-like (ARNTL), transcript variant 2 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: ARNTL aryl hydrocarbon receptor nuclear translocator-like [ Homo sapiens ]
Official Symbol: ARNTL
Synonyms: ARNTL; aryl hydrocarbon receptor nuclear translocator-like; aryl hydrocarbon receptor nuclear translocator-like protein 1; bHLHe5; BMAL1; JAP3; MOP3; PASD3; bHLH-PAS protein JAP3; member of PAS protein 3; member of PAS superfamily 3; brain and muscle ARNT-like 1; PAS domain-containing protein 3; ARNT-like protein 1, brain and muscle; basic-helix-loop-helix-PAS orphan MOP3; basic-helix-loop-helix-PAS protein MOP3; class E basic helix-loop-helix protein 5; TIC; BMAL1c; MGC47515;
Gene ID: 406
mRNA Refseq: NM_001030272
Protein Refseq: NP_001025443
MIM: 602550
UniProt ID: O00327
Chromosome Location: 11p15
Pathway: BMAL1:CLOCK/NPAS2 Activates Circadian Expression, organism-specific biosystem; Circadian Clock, organism-specific biosystem; Circadian Repression of Expression by REV-ERBA, organism-specific biosystem; Circadian rhythm - mammal, organism-specific biosystem; Circadian rhythm - mammal, conserved biosystem; Circadian rhythm pathway, organism-specific biosystem; Diurnally regulated genes with circadian orthologs, organism-specific biosystem;
Function: DNA binding; Hsp90 protein binding; RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity; aryl hydrocarbon receptor binding; sequence-specific DNA binding transcription factor activity; signal transducer activity;

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